Orion GenRead is a flexible benchtop solution including a small instrument and ready to use kits. It is intended for rapid detection of pathogens, offering a fast and accurate alternative for nucleic acid testing in various laboratory settings. Orion GenRead is based on Orion Diagnostica’s proprietary isothermal nucleic acid amplification technology, SIBA® (Strand Invasion Based Amplification)
SIBA® (Strand Invasion Based Amplification)
Isothermal nucleic acid amplification technologies offer significant advantages over polymerase chain reaction (PCR) in that they do not require thermal cycling or sophisticated laboratory equipment. However, non-target-dependent amplification has limited the sensitivity of isothermal technologies and complex probes are usually required to distinguish between non-specific and target-dependent amplification.
SIBA technology is able to detect a single molecule of the target analyte, and does not require target-specific probes. The technology relies on the recombinase-dependent insertion of an invasion oligonucleotide into the double-stranded target nucleic acid. The technology is resistant to non-specific amplification and is highly specific and able to distinguish closely-related species with single molecule sensitivity in the absence of complex probes or sophisticated laboratory equipment.
A scientific article on SIBA technology was published online in Plos One on 25 November 2014.
Strand Invasion Based Amplification (SIBA®): A Novel Isothermal DNA Amplification Technology Demonstrating High Specificity and Sensitivity for a Single Molecule of Target Analyte.