The interleukin-2 receptor (IL-2R), also known as CD25, is a transmembrane protein, which is up-regulated on activated T-cells, and shed into serum as soluble IL-2R, or sIL-2R. sIL-2R has emerged as an important biomarker that reflects T-cell activation and is often measured in serum to assess immune activity indicating disease status, progression or response to treatment. As serum levels can be increased in various inflammatory conditions, clinical context is required for accurate interpretation.

 

What sIL-2R indicates:

• T-Cell Activation: It's a key indicator of activated lymphocytes (T-cells), acting as a marker for immune response.

• Disease Activity: Elevated levels often signal active disease in immune-mediated conditions and cancers.

• Prognosis: High levels can predict poorer outcomes in certain cancers, like aggressive lymphomas, and severe inflammatory conditions. 

 

Common conditions where sIL-2R may be useful:

• Cancers: Haematological malignancy and lymphomas

• Inflammatory Diseases: Sarcoidosis, Rheumatoid Arthritis (RA), Systemic Lupus Erythematosus (SLE), Inflammatory Bowel Disease.

• Severe Immune Syndromes:  Haemophagocytic Lymphohistiocytosis (HLH). 

• Multiple Sclerosis (MS): Elevated levels in serum and CSF is indicative of disease activity in MS and strongly associated with relapsing-remitting MS (RRMS) relapses and progressive disease stages.

Measuring sIL-2R provides clinical teams with a sensitive biomarker for monitoring disease activity and treatment response, while research teams may gain valuable insight into T-cell dynamics in inflammatory conditions. 

 

What Is the TECAN-IBL sIL2R ELISA Kit? 

The sInterleukin-2-Receptor ELISA is intended for the quantitative determination of human soluble IL-2 receptor (sIL-2R) in serum and plasma (EDTA, citrate, heparin).

sIL-2R levels can be used as physiological marker for the indication of inflammation in the body, in-vivo immune system activation and response activity. As such the measurement of sIL-2R levels can be applied as an aid in the diagnosis or monitoring of therapy of autoimmune diseases (e.g. sarcoidosis).

The sInterleukin-2-Receptor ELISA is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the on the sandwich principle, and measured on an absorbance reader.

The assay is semi-automated requiring general purpose laboratory instruments and consumables such as absorbance microplate reader/washer, vortexer and pipettes to execute the test. The test kit is intended for manual use and can be adapted to different ELISA processors like TECAN EVOlyzer.

Test results are calculated from a standard curve and compared to laboratory established reference ranges from healthy adults (i.e. normal ranges).

 

How the sIL2R ELISA Supports Clinical & Research Teams 

• Ready-to-use format: Pre-prepared reagents, standards and internal controls reduce setup time and variability. 

• Traceable results: Calibrated to the NIBSC 97/600 reference standard, helping ensure reproducibility and comparability. 

• Reliable quality control: Built-in internal controls support consistent performance across experiments or clinical runs. 

• Flexible deployment: Compatible with manual ELISA workflows or automated systems, making it scalable for different lab needs. 

• Regulatory confidence: The kit is IVDR-compliant and registered with the MHRA in the UK, making it suitable for clinical use. 

 

Our Wider Product Range for Autoimmunity and Neurodegenerative diseases 

Oxford BioSystems supplies a broad range of immunoassay kits from several trusted manufacturers - including Biovendor, Mediagnost, Kamiya, TestLine, Diasource, and of course TECAN IBL. Each provides reliable kits for monitoring immune responses, tracking disease progression and supporting research into autoimmune and neurodegenerative diseases. For further information, visit our Immunology page, our Neurology page, or get in touch with a member of our customer services team.

 

Discover the IBL sIL-2R ELISA

If your clinical or research team is looking to monitor neuro-immune activation with confidence, the TECAN IBL sIL-2R ELISA offers a reliable, well-validated solution. 

For technical or pricing information, our team at Oxford BioSystems is here to help. 

 

Contact Oxford BioSystems today to find out more.