Autoimmune
The serological characterisation of autoimmune diseases is achieved by the detection of various autoantibodies or typical autoantibody profiles. The majority of these autoantibodies are directed against antigens located in the cells' nuclei, the nuclear plasma and matrix, but also against nucleolic, cytoplasmatic, mitochondrial or microsomal antigens
Rheumatoid diseases are mainly associated with the occurrence of anti-nuclear antibodies (ANA). Some of these ANA are found in several diseases, others are disease-specific and therefore can be used as serological markers in diagnosis. Antimitochondrial antibodies (AMA) against the M2 epitope typically react with mitochondrial proteins of the a-keto acid-dehydrogenase complexes and are characteristic markers for primary biliar cirrhosis, a chronic cholestatic liver disease.
Immunofluorescence
The earliest developed method for the detection of ANA and AMA has been the Immunofluorescence test (IFT) utilizing cryostatic tissue sections or cells as substrate.
For indirect immunofluorescence AID GmbH offer the ANA-HEp-2-Kit and ANA-HEp-2-Slides. Utilisation of HEp-2 cells, a human Larynx Epithelioma cell line with high specificity for most of the human ANA and ENA antibodies, circumvents the limitation of substrates of animal origin that are not suitable for the detection of several autoantibody specificities.
To place an order for the ANA-HEp-2-Kit, IF200e and/or the ANA-Hep-2-Slides IF210e, please contact us.
Immunoblots
AID GmbH provide a number of immunoblot products including:
ANA/AMA IgG Immunoblot ANA LineBlot Anti-Liver Autoantibody IgG Immunoblot
All immunoblots can be read using their unique scanning system for the acquisition, storage and evaluation of immunoblots (western blots) and DNA-stripes. The Immunoblot Scanning system omits the need to store blots physically and increases the comparability and reproducibility between blots.
For more information on the Immunoblot Scanning System please contact us.
ANA/AMA IgG Immunoblot
The ANA/AMA Immunoblot provides detection of antinuclear antibodies (ANA) and antimitochondrial antibodies (AMA).
Testing parameters include:
|
AMA (M2)
|
ACA (Centromer)
|
Histone
|
|
Jo-1
|
Ku
|
NuMA
|
|
PM-Scl
|
U-1 RNP
|
RNP (a and c)
|
|
Rib-P (rRNP)
|
Scl-70
|
Sm B’B
|
|
Sm D
|
SSA/Ro
|
SSB/La
|
The antigen is a nuclear extract from HEp-2 cells, which has been enriched with cytoplasmic proteins. For further information and clinical interpretation on the extractable nuclear antigens detectable in the ANA/AMA Immunoblot please visit AID-diagnostika.com.
To place an order for this product, IB100, please contact us.
ANA LineBlot
A new product to the immunoblot portfolio to further assist the diagnosis of autoimmune diseases. The highly sensitive and specific ANA LineBlot incorporates 12 recombinant, highly purified antigens on one strip to provide a supplement to the frequently used ANA/AMA Immunoblot. Testing parameters include:
|
dsDNA
|
SmB'B
|
U1-68/70 kD
|
|
RNP-A
|
rib.RNP
|
Ro-Ag
|
|
La-Ag
|
Sci-70
|
PM-Scl
|
|
ACA
|
Jo-1
|
|
The quick and easy to use ANA LineBlot is available as a 12 strip kit with ready to use regents.
To place an order for this product, DB100E, please contact us.
Anti-Liver Autoantibody IgG Immunoblot
The Anti–Liver Autoantibody Immunoblot utilises liver extract and provides detection of antibodies to the following:
AMA (M2), LKM Liver kidney microsomal antigen, LP Liver pancreas antigen
For further information on the specificity and clinical interpretation of the antigens detectable in the Anti-Liver Autoantibody Immunoblot please visit AID-diagnostika.com.
To place an order for this product, IB150, please contact us.
TNF-α blocker / inhibitor ELISA
TNF-α promotes the inflammatory response, which in turn causes many of the clinical problems associated with autoimmune disorders, such as rheumatoid arthritis, Crohn's disease and psoriasis.
These disorders are sometimes treated by using a TNF-α blocker or inhibitor, in the form of a therapeutic antibody such as infliximab (REMICADE®), adalimumab (HUMIRA®) and etanercept (Enbrel®).
There is the possibility that patients under anti TNF-α therapy may develop antibodies against the therapeutic antibodies, which limits the efficacy of the therapy and can lead to severe complications.
Immundiagnostik AG have a range of CE marked ELISA assays for use with serum and plasma samples to monitor anti TNF-α therapy by:
- determining levels of free therapeutic anti -TNF-α antibodies
- determining antibodies against the therapeutic anti TNF-α antibodies
|
K9650
|
Antibodies against TNFα- Blocker (ADA) ELISA kit (antibodies against e.g. REMICADE®)
|
|
K9652
|
Antibodies against TNFα- Blocker (ADA) ELISA kit (antibodies against e.g. HUMIRA®)
|
|
K9653
|
Antibodies against TNFα- Blocker (ADA) ELISA kit (antibodies against e.g. Enbrel®)
|
|
K9655
|
TNFα- Blocker-Monitoring ELISA kit (drug-level e.g. REMICADE®)
|
|
K9657
|
TNFα- Blocker-Monitoring ELISA kit (drug-level e.g. HUMIRA®)
|
For more information or to place an order please contact us.