Two-Tailed RT-qPCR
Patented Two-Tailed RT-qPCR technology with a unique mechanism of RT-primers to provide superior performance with excellent specificity in comparison to other methods. Instead of using a single binding probe, Two-tailed PCR uses two hemiprobes, which bind to different stretches of the microRNA, that are connected by a folded tether. Whilst each hemiprobe is too short to bind the microRNA, when both hemiprobes are complementary they bind cooperatively. Binding is exceedingly specific, as a mismatch is much more profound in a short hemiprobe.
Two-Tailed RT-qPCR is standardised for miRNA and piRNA total quantification.
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miREIA -miRNA enzyme immunoassay
miREIA is a novel, immunoassay-based method of miRNA quantification which involves the hybridisation of miRNA isolated from a patient sample to complementary biotinylated DNA oligonucleotide probe. The DNA/RNA hybrids are then captured by a microtiter plate-immobilised monoclonal antibody specific to perfectly matched DNA/miRNA hybrids. Development follows standard ELISA protocols.
The assay can be run on generic immunoassay equipment, does not require amplification steps and results are obtained in less than three hours including miRNA profiling.
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miRNA-RT-qPCR-MiRXES
MiRXES assays are designed in silico using proprietary 30-parameter thermodynamics-based algorithms and are extensively validated using both synthetic miRNAs and RNA from biological samples. Users can select from a growing library of validated human, mouse, rat, and viral miRNA assays.
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